Fig. 11

Inhibition of HMGA2/mTOR pathway increased drug sensitivity of SGC-7901/5-FU and SGC-7901/CDDP cells. The cell viability of SGC-7901/5-FU (a and c) and SGC-7901/CDDP (b and d) cells was determined after cells were transfected with siHMGA2/NC together with pEX-HMGA2-WT/MUT43 plasmid vectors (a and b) or treated with or without rapamycin (10 μM) (c and d) and then treated with 5-FU (a and c) or CDDP (b and d) for 48 or 24 h, respectively. Cell viability was determined by MTT assay. Cells treated with vehicle serve as a blank control. All experiments were conducted in quintuplicates and data were expressed as the mean ± SD (n = 5). Statistical significances were determined using one-way ANOVA followed by Dunnett’s test. ^*P < 0.05, ^**P < 0.01, compared with negative control transfection/DMSO group; ^#P < 0.05, ^##P < 0.01, compared with siHMGA2 transfection group; ns, no significance (P > 0.05), compared with the negative control transfection and siHMGA2 transfection groups