Fig. 4
From: MMP12 knockout prevents weight and muscle loss in tumor-bearing mice
IL-6 Up-regulates MMP12 in Macrophages. A Schematic diagram of tumor cells (MC38/CT26 cell lines) co-cultured with macrophage cells (RAW264.7 cell lines). All quantifications were performed by image J software for gray scale statistics. B, C Representative western blots showing the secreted MMP12 protein levels from RAW264.7 cell lines (1-2 × 105) cultured alone or co-cultured with MC38 cell lines (control, 1 × 104, 3 × 104, 5 × 104). β-Actin as the internal control. D, E Representative western blots showing the secreted MMP12 protein levels from RAW264.7 cell lines (1-2 × 105) cultured alone or co-cultured with CT26 cell lines (control, 1 × 104, 3 × 104, 5 × 104). GAPDH as the internal control. F Schematic diagram of IL-6 treated macrophages. RAW264.7 cells incubated with fresh media were served as untreated negative controls. Western blot was used to detect MMP12 in RAW 264.7 cells and GAPDH as the internal control. G, H RAW264.7 cells (1-2 × 105) were seeded in 6-well plates and treated with increasing doses of IL-6 (0, 2, 5, 10, 30 ng/mL) for 72 h. I Immune Gene data (https://www.immgen.org/ImmGenApps.html) suggest that IL-6 receptor expresses on F480+ macrophages. The colored bars refer to the expression level of IL-6 receptors on macrophages. Red represents high expression of IL-6 receptors, and green represents low expression of IL-6 receptors