Fig. 2

The nociceptor marker calcitonin gene-related peptide (CGRP) was correlated with both PNI and LNM in OSCC patients. A Kaplan–Meier survival analysis of cancer patients stratified by different neuropeptide mRNA levels according to the TCGA database. B Protein expression of CGRP in tumor tissues and adjacent normal tissues in PNI(−) and PNI(+) OSCC tissues. C Representative IHC images of OSCC tissues with different CGRP staining intensity. D Representative IHC images of PNI tissue from OSCC patients stained for CGRP and the nerve marker PGP9.5. E Quantification of CGRP scores in PNI(−) and PNI(+) OSCC tissues. F Representative immunohistochemistry images of a metastatic lymph node stained for CGRP. G CGRP staining levels in LNM(−) and LNM(+) OSCC tissues. BRCA, Breast invasive carcinoma; COAD, Colon adenocarcinoma; ESCA, Esophageal carcinoma; GBM, Glioblastoma multiforme; HNSCC, Head and Neck squamous cell carcinoma; LGG, Brain Lower Grade Glioma; LUSC, Lung squamouscell carcinoma