Fig. 5

EI24 was directly targeted by miR-455-3p and regulated by hsa_circ_0043278. A Prediction of miR-455-3p binding sites in EI24 and hsa_circ_0043278 using TargetScan and MiRanda. The binding sites are presented as solid lines. B Verification of the relative expression of EI24 in BC tissues (Tumour) and matched normal tissues (Normal) by qRT–PCR (n = 50). The middle horizontal lines in the scatter plot indicate the medians. C Pearson correlation analysis of EI24 and hsa_circ_0043278 expression in BC tissues (n = 50). D Relative mRNA levels of EI24 in cell lines were determined by qRT–PCR. E qRT–PCR and (F) western blot analysis of the EI24 mRNA level in MDA-MB-231 cells and MCF-7 cells after upregulation or downregulation of hsa_circ_0043278. (The images of the western blot bands were cropped from the images shown in Figs. S3 and S4). G The relative protein level of NF-κB (P65) in the cytosol and nucleus of cells in each group were determined by western blot analysis. (The images of the western blot bands were cropped from the images shown in Figs. S5, S6, S7, and S8.) The data are presented as the mean ± standard deviation values (n = 3); **** P < 0.0001