Fig. 5

XRCC1 knock-down induced apoptosis in MiaPaCa2 cells. A. MiaPaCa2 cells were treated with β-lap (6 μM/ 3 μM) for 2 h and then analysis of cell death in MiaPaCa2 cells (control and XRCC1 knocked-down) was performed at 24 h posttreatment by flow cytometry. B. Quantitation of the percentage of apoptotic & necrotic cells using the Annexin V assay. Values on the graph represent mean ± SD of three independent experiments. *** p ≤ 0.001; ** p ≤ 0.01; * p ≤ 0.05. (C) Western blot analysis of cleaved apoptotic & necrotic proteins in β-lap–treated XRCC1 knocked-down and control MiaPaCa2 cells