Fig. 1

β-lap induced lethality in NQO1⁺ PDA Cells. A. NQO1 expression were measured in Pancreatic cancer cells (MiaPaCa2, BxPC3, HS766T, or S2–013 cells). B-E. PDA cells (MiaPaCa2, BxPC3, HS766T, or S2–013 cells) were treated with or without various β-lap doses (μmol/L, 2 h), ± dicoumarol (50 μmol/L, 2 h). Lethality was monitored by relative survival. Control cells were treated with identical DMSO concentrations (< 0.05%). F. MiaPaCa2 cells knocked out for NQO1 expression (see Western, inset) were treated with β-lap as in (B) and survival were assessed. G. NQO1⁺ S2–013 cells harboring a CMV-NQO1 over-expression vector (see Western, inset) were treated as in (F) and survival were assessed. All error bars are means of six replicates from three independent experiments; means ± SEM. *** p < 0.001; ** p < 0.01; * p < 0.05