Fig. 7
From: The genotypic and phenotypic impact of hypoxia microenvironment on glioblastoma cell lines
Increased anti-apoptotic and proliferation markers under hypoxia. A & C. Cells were cultured under normoxia (N) and another set in hypoxia (H) for 72 h and stained for Survivin and Bcl2 by immunocytochemistry. The Images were taken with DMi8 leica microscope and prepared and quantified using ImageJ. The relative expression was normalized to tubulin. The data was analyzed using student t test by graph pad prism. B. Western blotting was done by the SDS-PAGE method and densitometry done using ImageJ. The relative expression was normalized to tubulin. The graphs were drawn and analyzed using t test by graph pad prism. Each value represents the mean ± SD of three independent experiments, * indicates p < 0.05. Scale bar = 50 μm. Representative Western blots are shown as cropped images. Full-length blots are presented in Additional File 5 (Fig. S5)