Fig. 2
From: The genotypic and phenotypic impact of hypoxia microenvironment on glioblastoma cell lines
Translocation of HIF-1α into the nucleus after the hypoxia acquisition. GBM cell lines were cultured under normoxia (N) and another set-in hypoxia (H) for 72 h and stained using HIF-1α by immunocytochemistry. The Images were taken with DMi8 Leica microscope and prepared and quantified using ImageJ graph pad prism. Analysis was done using student t test to compare the expression of HIF-1α between each cell line under normoxia verses hypoxia. Each value represents the mean ± SD of three independent experiments, * indicates p < 0.05. Scale bar = 50 μm