Fig. 2
CT-26 tumors are infiltrated with more anti-tumor immune cells than Colon 26 tumors. BALB/c mice were inoculated with 3 × 105 CT-26 or Colon 26 cells. When the tumor volume reached approximately 100 mm3, tumor tissues were harvested, dissociated into single cells and analyzed by flow cytometry. All data are represented as percent of total live cells, which include both tumor cells and immune cells. Quantification of (A) CD45+ cells, (B) T cells (CD45+ CD3+ CD11b-), (C) dendritic cells (CD45+ CD11b + CD11c + MHCII+), (D) NK cells (CD45+ CD3- CD11b- CD335+), (E) CD8+ T cells (CD45+ CD3+ CD11b- CD4- CD8+), (F) CD4+ T cells (Non-Tregs, CD45+ CD3+ CD11b- CD4+ CD8-Foxp3-), (G) Tregs (CD45+ CD3+ CD11b- CD4+ CD8- CD25+ Foxp3+), (H) ratio of CD8+ T cells to Tregs, (I) PD-1+ CD8+ T cells and (J) CTLA-4+ Tregs. Mean of each immune population are indicated as bars. Statistical significance (t-test) between groups: * 0.01 < p < 0.05, ** 0.001 < p < 0.01, *** p < 0.001. n.s., nonsignificant