Table 3 Temozolomide specification and methods for measuring cell viability
Characteristics | N = 212 |
|---|---|
Temozolomide concentration (μM) | |
Lower range (IQR)a | 0 (0-10) |
Upper range (IQR)a | 800 (250.0-1266) |
Unspecified | 30 (14.2%) |
Temozolomide exposure time | |
<24 hours | 8 (3.8%) |
24 hours | 24 (11.3%) |
48 hours | 34 (16.0%) |
72 hours | 59 (27.8%) |
96 hours | 15 (7.1%) |
>96 hours | 25 (11.8%) |
Range of time | 38 (17.9%) |
Unspecified | 9 (4.2%) |
Cell viability assay | |
MTT | 95 (44.8%) |
CCK-8 | 35 (16.5%) |
MTS | 11 (5.2%) |
Trypan blue | 11 (5.2%) |
Other | 55 (25.9%) |
Unspecified | 5 (2.4%) |
Method for cell viability quantification | |
Plate reader | 124 (58.5%) |
Flow cytometry | 13 (6.1%) |
Microscopy and manual counting | 11 (5.2%) |
Microscopy and automated counting | 3 (1.4%) |
Technique in a reference/manual | 16 (7.5 %) |
Unspecified | 45 (21.2%) |