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Table 2 Experimental design of 212 included studies

From: Temozolomide sensitivity of malignant glioma cell lines – a systematic review assessing consistencies between in vitro studies

Characteristics N = 212
Base medium
 DMEM 141 (66.5%)
 Customised media 23 (10.8%)
 Eagle minimal essential medium 18 (8.5%)
 DMEM/F12 11 (5.2%)
 Neurobasal 2 (0.9%)
 Unspecified 17 (8.0%)
Serum supplementation
 Yes 190 (89.6%)
 No 1 (0.4%)
 Unknown 21 (9.9%)
Carbon dioxide (%)
 5 156 (73.6%)
 6 1 (0.5%)
 10 1 (0.5%)
 Unknown 54 (25.5%)
Temperature
 37°C 160 (75.5%)
 Unknown 52 (24.5%)
Hypoxic condition
 Used 5 (2.4%)
 Unknown 54 (25.5%)
Other methodological reporting
 Cell density 154 (72.6%)
 Number of cell passage 16 (7.5%)
 Number of replicates 58 (27.4%)
 Number of plate replicates 42 (19.8%)
  1. DMEM Dulbecco’s Modified Eagle Medium, DMEM/F12 Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12