Table 2 Experimental design of 212 included studies
Characteristics | N = 212 |
|---|---|
Base medium | |
DMEM | 141 (66.5%) |
Customised media | 23 (10.8%) |
Eagle minimal essential medium | 18 (8.5%) |
DMEM/F12 | 11 (5.2%) |
Neurobasal | 2 (0.9%) |
Unspecified | 17 (8.0%) |
Serum supplementation | |
Yes | 190 (89.6%) |
No | 1 (0.4%) |
Unknown | 21 (9.9%) |
Carbon dioxide (%) | |
5 | 156 (73.6%) |
6 | 1 (0.5%) |
10 | 1 (0.5%) |
Unknown | 54 (25.5%) |
Temperature | |
37°C | 160 (75.5%) |
Unknown | 52 (24.5%) |
Hypoxic condition | |
Used | 5 (2.4%) |
Unknown | 54 (25.5%) |
Other methodological reporting | |
Cell density | 154 (72.6%) |
Number of cell passage | 16 (7.5%) |
Number of replicates | 58 (27.4%) |
Number of plate replicates | 42 (19.8%) |