Fig. 3
From: Identification of TRPV4 as a novel target in invasiveness of colorectal cancer
TRPV4 increases ZEB1 expression to enhance epithelial-mesenchymal transition. A and B Western blot analysis for p-AKT, AKT, ZEB1 and ATCB in HCT-116 and SW620 cells transfected with siCTL and siTRPV4#1 or treated with Vehicle or HC-067047. C and D representative images and summary data of Western blot demonstrating the effects of ZEB1 siRNA or AKT siRNA on the EMT process induced by TRPV4 overexpression in HT-29 cells. E and F representative images and summary data from migration and invasion assay demonstrating the effects of ZEB1 siRNA on TRPV4 overexpression induced cell motility, scale bar: 50 μm. G and H representative images and summary data from migration and invasion assay demonstrating the effects of AKT siRNA on TRPV4 overexpression induced cell motility, scale bar: 50 μm. Values represent the mean ± SEM, #, p < 0.05 compared to siCTL, &, p < 0.05 compared to Vehicle, *, p < 0.05 compared to TRPV4