Fig. 2
From: Identification of TRPV4 as a novel target in invasiveness of colorectal cancer
![Fig. 2](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs12885-021-08970-7/MediaObjects/12885_2021_8970_Fig2_HTML.png)
TRPV4 promotes colon cancer cell migration and invasion through regulating epithelial-mesenchymal transition process. A Western blot analysis of TRPV4 showing increased TRPV4 levels in HT-29 cells transfected with a TRPV4-overexpression construct. B TRPV4 mRNA levels in HT-29 cells transfected with a TRPV4-overexpression construct. C and D representative images and summary data of migration and invasion assay in HT-29 cells transfected with a TRPV4-overexpression construct, scale bar: 50 μm. E and F RNA levels of the epithelial-mesenchymal transition (EMT) markers in SW620 cells transfected with siCTL, siTRPV4#1 and siTRPV4#2 or treated with Vehicle or HC-067047. G Western blot analysis for EMT markers showing a decreased EMT process in SW620 cells transfected with siCTL, siTRPV4#1, and siTRPV4#2 or treated with Vehicle or HC-067047. H Western blot analysis for EMT markers showing an increased EMT process in HT-29 cells transfected with a TRPV4-overexpression construct. I Summary data for A. J Summary data for G. K Summary data for H. Values represent the mean ± SEM, #, p < 0.05 compared to siCTL or Vehicle or Vector