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Fig. 2 | BMC Cancer

Fig. 2

From: Identification of TRPV4 as a novel target in invasiveness of colorectal cancer

Fig. 2

TRPV4 promotes colon cancer cell migration and invasion through regulating epithelial-mesenchymal transition process. A Western blot analysis of TRPV4 showing increased TRPV4 levels in HT-29 cells transfected with a TRPV4-overexpression construct. B TRPV4 mRNA levels in HT-29 cells transfected with a TRPV4-overexpression construct. C and D representative images and summary data of migration and invasion assay in HT-29 cells transfected with a TRPV4-overexpression construct, scale bar: 50 μm. E and F RNA levels of the epithelial-mesenchymal transition (EMT) markers in SW620 cells transfected with siCTL, siTRPV4#1 and siTRPV4#2 or treated with Vehicle or HC-067047. G Western blot analysis for EMT markers showing a decreased EMT process in SW620 cells transfected with siCTL, siTRPV4#1, and siTRPV4#2 or treated with Vehicle or HC-067047. H Western blot analysis for EMT markers showing an increased EMT process in HT-29 cells transfected with a TRPV4-overexpression construct. I Summary data for A. J Summary data for G. K Summary data for H. Values represent the mean ± SEM, #, p < 0.05 compared to siCTL or Vehicle or Vector

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