Fig. 3

SET is a direct target of miR-1915-3p. A Target candidates of miR-1915-3p were predicted using four bioinformatic tools, Targetscan, miRDB, miRanda and miRwalk. B Relative mRNA expression of each target candidates was detected by qRT-PCR in the miR-1915-3p overexpression A549 cells. C Schematic depicting the construction of the WT or deletion (Del) SET 3′UTR vectors. D, E qRT-PCR and western blot analysis of endogenous SET expression in A549 and H1975 cells transfected with miR-NC or miR-1915-3p mimic. F, G Endogenous SET expression was determined both at mRNA and protein levels in anti-miR-1915-3p-transfected NSCLC cells. H Dual-luciferase assay was performed in A549 and H1975 cells and showed a direct binding association between SET and miR-1915-3p. Each bar represented the means±SEM for triplicate experiments. p values were evaluated by Student’s t-test. *p < 0.05, **p < 0.01, ***p < 0.001. NS, no significant. I The relationship between SET and miR-1915-3p expression was assessed by Spearman’s rank correlation analysis