Fig. 5

Blockade of HIF1α improved outcome of chemotherapy in osteosarcoma. A The tumor growth of the Saos-2 tumor was detected after treatment with DMSO, MTX (5 mg/kg), KC7F2 (10 mg/kg) and MTX combined with KC7F2. B The tumor growth of the Saos-2 tumor was detected after treatment with DMSO, CIS (1 mg/kg), KC7F2 (10 mg/kg) and CIS combined with KC7F2. C The survival time of the Saos-2 tumor was detected after treatment with DMSO, MTX (5 mg/kg), KC7F2 (10 mg/kg) and MTX combined with KC7F2. D The survival time of the Saos-2 tumor was detected after treatment with DMSO, CIS (1 mg/kg), KC7F2 (10 mg/kg) and CIS combined with KC7F2. E Osteosarcoma cells from chemo-resistant patients were cultured in 3D matrix gel. Colony size was indicated after 3 (D3) and 10 days (D10). The scale bar is 10 μm. F Osteosarcoma cells from chemo-resistant patients were cultured in 3D matrix gel and treated with MTX (75 mM) plus KC7F2 (20 μM). Cell apoptosis was determined by flow cytometry. G The same as F except that osteosarcoma cells were treated with CIS (40 μM) plus KC7F2 (20 μM). MTX, methotrexate; CIS, cisplatin