Fig. 2

USP3 and Aurora A promote proliferation of ESCCcells. A-B Aurora A and USP3 were knocked down by siRNA in ESCCcell line ECa109 cells, respectively, and knockdown efficiency was measured by qPCR. C-D Aurora A and USP3 were knocked down by siRNA in ECa109 cells, and the protein was knocked out in the cells by western blot. E-F Overexpression of Aurora A and USP3 in ECa109 cells, and Western blot analysis confirmed that proteins were highly expressed in cells. G-J CCK8 confirmed that Aurora A and USP3 promoted the proliferation of ECa109 cells. K-L After Aurora A and USP3 knockdown by siRNA in ECa109 cells, cells were plated in 12-well plates, cultured for 14 days, and then stained with crystal violet. M-N After over-expressing Flag-Aurora A and HA-USP3 in ECa109 cells, cells were plated in 12-well plates, cultured for 14 days, and then stained with crystal violet