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Fig. 3 | BMC Cancer

Fig. 3

From: RETRACTED ARTICLE: FAM83H-AS1/miR-485-5p/MEF2D axis facilitates proliferation, migration and invasion of hepatocellular carcinoma cells

Fig. 3

FAM83H-AS1 indirectly regulates MEF2D expression in HCC cells. (A) The binding ability of miR-485-5p with candidate mRNAs was tested via RNA pull-down assay. (B-C) MEF2D expression in different transfected cells was measured via RT-qPCR analysis. (D) MEF2D expression in HCC cell lines and THLE-3 cell line was examined by RT-qPCR analysis. (E) Evaluation of MEF2D overexpression efficiency was conducted via RT-qPCR. (F) The luciferase activity of FAM83H-AS1, miR-485-5p or MEF2D was detected in the indicated transfected HCC cells. (G) The inhibition efficiency of miR-485-5p inhibitor was verified via RT-qPCR. (H) MEF2D expression in different groups was examined by RT-qPCR analysis. **P < 0.01

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