Fig. 3

Aberrant activation of Stat5a downregulates the promoter transcriptional activity of Dnmt3a. A. Segments of the promoter region of the Dnmt3a gene. B & C. Incubation with LY2784544 significantly increased the fluorescence intensity of 293 T cells transfected with the P4 region luciferase plasmid. D. The P4 region promoter transcriptional activity was strongest after 3 h of LY2784544 incubation. E. Stat5a was overexpressed in 293 T cells, and IL-3 was added to simulate the abnormal activation and phosphorylation of Stat5a. Full-length blot images are presented in Supplementary Fig. 13. F. Overexpression and activation of Stat5a inhibited the transcriptional activity of the P4 region promoter. G. The effect of LY2784544 on transcriptional activity of the Dnmt3a promoter with or without mutated GAS