Fig. 1
Aberrant activation of the JAK-STAT pathway represses Dnmt3a transcription and translation via Stat5a. A. The expression level of Jak2, p-Jak2, Stat5a, p-Stat5a and Dnmt3a in wild-type and Jak2V617F BaF3 cells. Full-length blot images are presented in Supplementary Fig. 1. B. Transcription level of DNMT3a in bone marrow mononuclear cells from JAK2V617F MPN patients and normal controls. C. p-Stat5a and Dnmt3a of Jak2V617F Baf3 cells incubated with AZ960. Full-length blot images are presented in Supplementary Fig. 2. D. p-Stat5a and Dnmt3a of Jak2V617F Baf3 cells incubated with LY2784544. Full-length blot images are presented in Supplementary Fig. 3. E. In Jak2V617F BaF3 cells, the protein expression level of Dnmt3a after Stat5a was knocked down. Full-length blot images are presented in Supplementary Fig. 4. F. The transcriptional level of Dnmt3a in Jak2V617F Baf3 cells with or without LY2784544 incubation