Fig. 2

The expression of miR-16-5p and ANLN and verification of miR-16-5p’s target ANLN. a-c ANLN was highly expressed in BC compare with normal tissues from the data of GSE29431, GSE42568 and TCGA. d-e qRT-PCR was used to explore miR-16-5p and ANLN in BC and normal cell lines. f-g The dual-luciferase reporter assay was used to prove the relationship between miR-16-5p and ANLN. qRT-PCR (h-i) and western blot (j-k) was used to investigate ANLN and its protein expression after transfection of miR-16-5p mimcis, si-ANLN, NC or both in MCF-7 and MDA-MB 231 cells. l-m BC cell proliferation was detected by MTT assay. Data represent the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 compared with the NC group. BC, breast cancer; NC, negative control