Fig. 4

GLRX3 regulates c-Met signaling in PDAC cells: A Flow cytometry analysis of c-Met expression. Histograms indicate c-Met-FITC positive cells. c-Met positive cells were reduced upon GLRX3 knockdown (red) compared to the control (blue); B In western blot analysis, c-MET and its downstream signal molecules were downregulated by shRNA transfection in HPAC cells; C The effect of GLRX3 or Met silencing on Met/PI3k/AKT singling in HPAC cells. The expression of c-Met, PI3K, and phosphorylation of AKT was reduced by siRNA transfection; D Immunohistochemical analysis of mouse tumor tissues from CFPAC-1 NC and GLRX3 K/D cells (3B10 clones) revealed cMET expression was significantly decreased in GLRX3 K/D tumor than in control; E Glrx3 and Met mRNA expression levels in pancreatic cancer tissues were downloaded from the TCGA by using ISB Cancer Genomics Cloud. Outlier samples were removed by clipping the top and bottom deciles of each expression distribution. The correlation between GLRX3 and Met mRNA expression is illustrated as a scatter plot where each dot represents a single cancer tissue sample. The Pearson’s correlation value (R), and the p value are indicated; F The Glrx3 and Met mRNA expression and cancer patients’ clinical data were derived from the TCGA database. Patients were divided into low (red line) or high (blue line) expression groups, using maximally selected rank statistics. Survival of patients was visualized using the Kaplan–Meier plot. The log-rank p value between the groups are shown in each plot