Fig. 6

MiR-490-3p targeted AURKA in bladder cancer cells. A TargetScan showed the predicted binding sequences of AURKA for miR-490-3p. B Dual-luciferase assay was performed in cells co-transfected with plasmids AURKA 3′UTR-Wt or AURKA 3′UTR-Mut and NC mimics or miR-490-3p mimic in RT4 and T24 cells. C Enrichment of miR-490-3p and AURKA analyzed by RNA pull-down assay. D RT-qPCR detection of AURKA expression in bladder cancer tissues (n = 34) and normal tissues (n = 34). E The correlation between the relative expression level of miR-490-3p and AURKA. F RT-qPCR detection of AURKA expression in SV-HUC-1, RT4, and T24 cell lines. G Measurement of AURKA protein expression in RT4 and T24 cells transfected with NC, Si-AURKA, inhibitor, and Si-AURKA+inhibitor. ^*, P < 0.05; ^**, P < 0.001 compared to blank. ^#, P < 0.05; ^##, P < 0.001 compared to Si-AURKA +inhibitor. NC, negative control; Wt, wild-type; Mut, Mutant; Si-AURKA, siRNA-AURKA; inhibitor, miR-490-3p inhibitor; Si-AURKA+inhibitor, siRNA-AURKA+ miR-490-3p inhibitor