Fig. 4
BZM attenuated MTX-induced apoptosis but enhanced anti-proliferation in vivo. A-D. Subcutaneous xenografts were established with LNCaP cells in male SCID mice and animals were treated with the vehicle (as control, n = 10), BZM (1 mg/kg), MTX (3 mg/kg) and combination of and MTX (1.5 mg/kg) and BZM (0.5 mg/kg). Mice bearing a tumor volume closing to 1500 mm3 were euthanized. Tumor tissues were fixed and performed IHC stain with indicated antibodies or TUNEL kit (n = 4, per group).A. Ki67 expression of tumor tissues. Representative IHC staining of Ki67, Scale bar, 50 μm. B. Quantitative data of Ki67 expression are presented from three independent experiments. C. Apoptosis were determined by TUNEL kit. Representative IHC staining of TUNEL, Scale bar 100 μm. D. Quantitative data of apoptosis analysis are presented from three independent experiments. All data are presented as mean ± s.e.m. The asterisk indicates a significant difference (one-way ANOVA, *p < 0.05; **p < 0.01; ***p < 0.001)