Fig. 3

BZM attenuated MTX-induced apoptosis. A-D. LNCaP (A, B) and 22RV1(C, D) cells were treated with the vehicle, BZM(200 nM), MTX(1 μM) or combination(BZM, 100 nM; MTX, 0.5 μM) for 24 h. Apoptosis analysis was performed using Annexin-V/PI staining approach coupled with flow cytometry. A, C. Representative flow cytometry plots. B, D. Quantitative data of apoptosis analysis are presented from three independent experiments. Early apoptotic was defined as Annexin positive population, late apoptotic as Annexin/PI positive. The asterisk indicates a significant difference (one-way ANOVA, *p < 0.05, **p < 0.01)