Skip to main content

Table 4 ICC-staining comparing control cells with cells after 12-h or 24-h exposure to 5 μM FX-9

From: Novel chemotherapeutic agent FX-9 activates NF-κB signaling and induces G1 phase arrest by activating CDKN1A in a human prostate cancer cell line

 

12-h

24-h

control

5 μM FX-9

control

5 μM FX-9

CDKN1A (p21)

intensity

m

w-m

m

m

positively stained cells

+

+

++

+++

E2F1

intensity

w-m

m

w-m

w-m

positively stained cells

+++

+++

++

++

SERPINE1 (PAI-1)

intensity

w

w

w

w

positively stained cells

+++

++++

++++

++++

NFKB2

intensity

m-s

s

w-m

m-s

positively stained cells

++++

++++

++++

++++

  1. Intensity of ICC-staining is categorized into negative (n); weak (w); moderate (m); strong (s). The fraction of positively stained cells are categorized into four groups: + = 0–10% of labeled cells; ++ = 11–40% of labeled cells; +++ = 41–70% of labeled cells; ++++ = 71–100% of labeled cells. Images of ICC-stainings are included into supplementary information (supplementary Figs. 1 and 2).