Fig. 4

MiR-545-5p mimics inhibited the proliferation, migration and invasion of GC cell line AGS might through CNPY2. A-D AGS cells were transfected with miR-545-5p mimics or NC mimics. Cell proliferation was evaluated by CCK-8 assay (A) and colony formation assay (B). C Cell migration was evaluated by wound healing assay. D Cell invasion was evaluated by Transwell assay. E The putative binding site between miR-545-5p and CNPY2 was predicted by Targetscan. F The relative luciferase activity of CNPY2 WT/MT was detected by dual luciferase reporter system. G AGS cells were transfected with overexpressing plasmid (LINC00342) and the empty vector, and the transfection efficiency was confirmed by qRT-PCR. (H-J) AGS cells were transfected with miR-545-5p mimics, or co-transfected with miR-545-5p mimics and overexpressing plasmid (LINC00342). The expression of CNPY2 was evaluated by qRT-PCR (H) and western blot (I). J The expression of CNPY2 was evaluated by immunofluorescence using anti-CNPY2 antibody. × 200 magnification, scale bar = 100 μm. * p < 0.05, ** p < 0.01 vs NC mimics/the empty vector; ## p < 0.01 vs miR-545-5p mimics + the empty vector