Fig. 6

Microcell viability detection using NRU assay in SK-MEL-28 cell line after applied PTX for 48 h. (A) Untreated SK-MEL-28 cells with positively NR uptake, NRU – NR uptake, PhC- phase contrasts, NRU/PhC - NRU and PhC overlay; (B)SK-MEL-28 cell treated with PTX with significant NR uptake in microcells (NRU, red arrow), NRU – NR uptake, PhC – phase contrast, NRU/PhC - NRU and PhC overlay. Microcell showed intensive NR uptake and this small single cell is localized nearby microcell debris (PhC). The scale bar is 20 μm