Fig. 4

CCNG2 inhibits cell cycle progress in HNSCC in vitro. (A) qRT-PCR was used to detect the intracellular expression level in HNSCC cell lines Tu686 and FaDu compared with HuLa-PC cells. *p < 0.05, ***p < 0.001 vs. HuLa-PC cells. (B) qRT-PCR and (C) western blot were used to detect the CCNG2 mRNA and protein levels after plasmid transfection. ****p < 0.0001 vs. pcDNA3.1 group. Gray value analysis is shown below the band (normalized to GAPDH). (D) Tu686 cells and FaDu cells were transfected with CCNG2 plasmid or pcDNA3.1. Cell cycle phase distribution was detected by flow cytometry. Data shown are the mean ± standard deviation of three independent experiments. *p < 0.05, **p < 0.01 vs. pcDNA3.1 group. (E) Cell cycle-related genes were detected by qRT-PCR. *p < 0.05, **p < 0.01 vs. pcDNA3.1 group