Fig. 2

Determining the appropriate number of cells and the sunitinib concentration required to generate sunitinib-resistance in SNU-228 and SNU-267 cells. Cell proliferation was measured for 72 h after seeding the cells at 500, 1000, 2000, 5000, 7000, and 10,000 cells/well in 96-well plates; A SNU-228 and B SNU-267 cells. The y-axis is the absorbance measured at 450 nm (A and B). The population of SNU-228 cells was measured after 1000 cells/well were seeded in 96-well plates and then exposed to a concentration gradient of sunitinib for 72 h (C). SNU-267 cells were tested in the same way after 2000 cells/well were seeded (D). The GI₅₀ values were then calculated. This experiment was conducted three times independently