Fig. 5

LINC00852 acts as a ceRNA to regulate AGTR1 expression by sponging miR-140-3p. a Bioinformatics software (Starbase) predicted that AGTR1 was a target of miR-140-3p. b The regulation of miR-140-3p on AGTR1 was confirmed by dual luciferase reporter gene assay. *p < 0.05, compared with mimic NC. c LINC00852, miR-140-3p and AGTR1 expressions were detected in different groups of SKOV-3 cells by qRT-PCR. *p < 0.05, compared with oe-LINC00852 + mimic NC. d Protein levels of MEK, p-MEK, ERK1/2, p-ERK1/2, STAT3 and p-STAT3 were detected in different groups of SKOV-3 cells by Western blotting. *p < 0.05, compared with oe-LINC00852 + mimic NC. e AGTR1 expression level was detected in normal human ovarian epithelial cells IOSE80 and ovarian cancer cells (A2780, SKOV-3, OV-90 and CAOV3). *p < 0.05, compared with IOSE80. f-g In in vivo model, tumor volume was detected in sh-AGTR1 group and sh-NC group. h Tumor weight was measured in sh-AGTR1 group and sh-NC group. *p < 0.05, compared with sh-NC