Fig. 6

Apoptosis Induction by Alectinib and light treatment in HCT116 Bax^−/− / Bak^−/−. HCT116 wild-type (WT) cells and isogenic double knockout subline HCT116-Bax^−/−/Bak^−/− cells were treated with DMSO alone or 10 μM Alectinib/DMSO, 150 μM, and LED light. Combined treatment of cells with Alectinib and light induced cell death in both cell lines, but number of Annexin V-positive/PI-negative was significantly reduced and number of Annexin V-positive/PI-positive increased in the double knockout cells in comparison to HCT116 WT (Data show mean ± SD of three independent experiments; * p < 0.05). WB analysis (right side) confirmed loss of Bax and Bak expression in HCT116 double knock out cells and shows that FECH expression in HCT116-wt cells and isogenic HCT116-Bax^−/−/Bak^−/− cells is comparable. Level of FECH expression was normalized to respective actin control and presented as relative protein expression level in relation to respective wild type control. (Analysis was performed twice. Both WB including quantification and indication of the mean value are shown in the supplemental Fig. 2)