Fig. 4

Light treatment increased Alectinib-induced phosphatidylserine exposure of NSCLC and breast cancer cells. NSCLC cells H460, H1299 and MDA-MB-231 breast cancer cells were / or were not treated with 10 μM Alectinib, 150 μM ALA, and exposure to a LED lamp emitting light at a wavelength of 405 nm (X-axis). Phosphatidylserine exposure (Y-axis) was determined using an Annexin V-FITC detection kit by flow cytometry. Light treatment (black columns) increased the effect of Alectinib (right side of each figure) significantly. In H460, H1299 and MDA-MB-231 cell lines light treatment (black columns) increased the effect of Alectinib (right side of each figure) significantly (* p < 0.05). In contrast, in the non-tumorigenic human breast cell line MCF-10A the rate of cell death is not increased by additional LED light irradiation. (mean ± SD of three independent experiments; * p < 0.05)