Fig. 3
From: Alectinib treatment improves photodynamic therapy in cancer cell lines of different origin
Induction of phosphatidylserine exposure in breast cancer cells due to LED light treatment. Light induction of apoptosis in MDA-MB-231 breast cancer cells pretreated with 250 μM ALA. LED lamp emitting light at a wavelength of 405 nm was operated at 25 mA leading to an intensity of 4 mW/cm2. Duration of light treatment was between 20 min. and 180 min. (X-axis). Apoptosis was determined using an Annexin V-FITC detection kit by flow cytometry. Exposure of ALA-pretreated MDA-MB-231 breast cancer cells to LED light led to a rate of about 20% apoptotic cells. (Data show mean ± SD of three independent experiments; * p < 0.05)