Fig. 4

PRRX1 acts as tumour suppressor in HCC cell lines and affects ZEB1/2 expression. A PRRX1 knockdown in different HCC cell lines as determined by qPCR analysis. The knockdown was induced by transfection of 25 nM siPRRX1. Samples were taken after 48 h; bars indicate mean ± SD and are representative of 3 experiments, each in triplicate. B ZEB1 and ZEB2 expression as determined by qPCR 48 h after siPRRX1 knockdown. Bars indicate mean ± SD and representative of 3 experiments each in triplicates. C Cell migration scratch assay 24 h after siPRRX1 transfection. Bars indicate mean ± SD of gap distance (n = 18 equidistant measurements) after scratching the monolayer with a 200 μl pipette tip. D MTT proliferation assay as measured 48 h after siPRRX1 transfection. Experiment was repeated at least 3 times. Bars indicate mean ± SD, n = 12 per group (4 from each experiment). E Colony formation assay 8 days after PRRX1 knockdown. The experiment was repeated at least 3 times. CV = crystal violet. Bars show CV quantification mean values ± SD, n = 9 per group from one representative experiment