Fig. 5

NR1D1 inhibited proliferation, induced apoptosis and suppressed the STAT3 signal through up-regulating SOCS3. A-B The level of NR1D1 in ovarian cancer cell lines COC1, SK-OV-3, OVCAR-3, A2780 and normal ovarian epithelial cell line (NOEC) was determined by quantitative real-time PCR and western blot. C Positive correlation between NR1D1 and SOCS3 in ovarian cancer according to the data from TCGA (n = 426). D-E The mRNA level of SOCS3 was determined by quantitative real-time PCR after transfection with NR1D1 over-expression plasmid or shRNAs. F The protein level of SOCS3 was determined by western blot after transfection with NR1D1 over-expression plasmid or shRNAs. G The level of SOCS3 in tumors with NR1D1 over-expression was determined by western blot. H Transfection efficiencies of SOCS3 siRNAs were determined by western blot. I After co-transfection with NR1D1 over-expression plasmid and SOCS3 siRNA, the cell viability was determined with CCK-8. J Apoptosis was determined by flow cytometry after co-transfection with NR1D1 over-expression plasmid and SOCS3 siRNA. K The level of phosphorylated STAT3 was determined by western blot in cells co-transfected with NR1D1 over-expression plasmid and SOCS3 siRNA. The results are presented as mean + SD