Fig. 1

NR1D1 inhibited the proliferation of ovarian cancer cells. A Data from TCGA showed that NR1D1 has a low expression level in ovarian cancer tissues (ovarian cancer n = 426 and normal ovarian tissues n = 88). B Low NR1D1 level was correlated to the poor survival of ovarian cancer patients at advanced stage (n = 1145). C-D The level of NR1D1 in ovarian cancer cell lines COC1, SK-OV-3, OVCAR-3, A2780 and normal ovarian epithelial cell line (NOEC) was determined by quantitative real-time PCR and western blot. E Transfection efficiency of NR1D1 over-expression plasmid was determined by western blot. F After transfection with NR1D1 over-expression plasmid, the proliferation of ovarian cancer cells was determined with CCK-8. G The level of PCNA in ovarian cancer cells was determined by immunofluorescence after transfection with NR1D1 over-expression plasmid. Scale bar = 50 μm. H The transfection efficiencies of NR1D1 shRNAs in ovarian cancer cells were determined by western blot. I The proliferation of NR1D1 silenced cells was determined with CCK-8. (J) Immunofluorescence was performed to determine the level of PCNA in NR1D1 silenced cells. Scale bar = 50 μm. The results are presented as mean + SD