Fig. 2

IMR90 E6E7/E6E7 RST hybrid cells exhibit basal respiratory rates similar to RST parental cells. A Respiratory rate of E6E7, RST and H1-H6 hybrids under pyruvate. Values normalized to number of cells and expressed in nmol of O₂ consumed per minute for one million cells (n = 2 for H1, H5; n = 3 for E6E7, H2, H3, H4, H6; n = 6 for RST; each experiment done in duplicate). Statistical analyses were done using one-way ANOVA test followed by Holm-Sidak multiple comparison test (*p < 0.05, **p < 0.01; error bars, SD). B Citrate synthase activity was evaluated for two parental and all hybrid cells directly from samples used to determine respiration rate as described in (a). Statistical analyses were done using one-way ANOVA test followed by Holm-Sidak multiple comparison test (error bars, SD). C Citrate synthase normalized respiratory rate of E6E7, RST and H1-H6 hybrids under pyruvate. Each value obtained in (A) was normalized by citrate synthase activity found in the same sample. Statistical analyses were done using one-way ANOVA test followed by Holm-Sidak multiple comparison test (*p < 0.05, **p < 0.01; error bars, SD)