Fig. 3

Knockdown of CDC25A suppresses growth of xenograft tumor in mice. A ME180 and C33A cells with stable transfection of sh-CDC25A or sh-NC were subcutaneous injection into the flank of the NOD/SCID mice, and then the volume of xenograft tumors was evaluated every 5 d; B weight of xenograft tumors; C expression of Ki-67 in xenograft tumors determined by IHC staining; D number of apoptotic cells in xenograft tumors determined by TUNEL assay. N = 5 in each group. Data were presented as the mean ± SD from three independent experiments. Data were analyzed by two-way ANOVA followed by Tukey’s multiple comparison test. In all panels, **p < 0.01 vs. sh-NC