Fig. 1

CDC25A is highly expressed in cervical cancer. A volcano plots representing the DEGs in the GEO GS63514 dataset; B a heatmap for top 50 DEGs between 24 cases of normal cervical epithelial tissues and 104 cases of CSCC tissues; C relevance between CDC25A expression and CSCC grade according to the data in the GSE63514 chip; D CDC25A expression in TCGA-CESC (data of CESC tissues) and GTEx-Cervix (data of normal cervical tissues) in the GEPIA database; E-F mRNA (E) and protein (F) expression of CDC25A in CSCC cell lines (C33A, Caski, HeLa and ME180) and normal cervical epithelial cells (PCS480) determined by RT-qPCR and western blot analysis. Data were presented as the mean ± SD from three independent experiments. In panel D, data were analyzed by the unpaired t test, while in panels C, E and F, data were analyzed by one-way ANOVA followed by Tukey’s multiple comparison test. In panel C, Low grade/High grade vs. Normal, ***p < 0.001; Low grade vs. High grade, ***p < 0.001; in panel D, tumor tissues vs normal tissues, p < 0.05; in panels E and F, C33A/Caski/HeLa/ME180 vs. PCS480, **p < 0.01, ***p < 0.001. All cellular experiments were performed in triplicates and three duplicated wells were set