Fig. 2From: MARCH6 promotes hepatocellular carcinoma development through up-regulation of ATF2MARCH6 expression is essential for HCC cell growth. (a) Immunoblotting analysis of MARCH6 in HCCLM3, Huh7 and MHCC97H cells. (b) Immunoblotting analysis of MARCH6 in shCtrl, shMARCH6–1 and shMARCH6–2 MHCC97H cells, and in Ctrl and MARCH6 overexpressed Huh7 and HCCLM3 cells. (c) CCK-8 determined cell viability in shCtrl, shMARCH6–1 and shMARCH6–2 MHCC97H cells, and in Ctrl and MARCH6 overexpressed Huh7 and HCCLM3 cells. n = 3. (d and e) shCtrl, shMARCH6–1 and shMARCH6–2 MHCC97H cells, Ctrl and MARCH6 overexpressed Huh7 were subjected to EdU staining. n = 3. Scale bar, 50 um. ** P < 0.01, *** P < 0.001Back to article page