Fig. 6

FMNL2 promotes breast cancer cell proliferation partially by a p27-related mechanism. After transfection for 48 h, cultured cells were processed for indicated assays. a and b The expression of FMNL2, p27 and Ki67 was detected by western blotting. c MTT assay was performed at 48 h post-transfection. *: P < 0.05 vs NC siRNA group. ^#: P < 0.05 vs sip27 group. d and e The expression of FMNL2, p27, Lamin A/C and Ki67 was detected by western blotting. f MTT assay was performed at 48 h post-transfection. g Representative percentage of cells in the G0/G1, G2/M and S phases was detected by flow cytometric analysis. *: P < 0.05 vs p27WT vector group