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Fig. 3 | BMC Cancer

Fig. 3

From: Formin-like protein 2 promotes cell proliferation by a p27-related mechanism in human breast cancer cells

Fig. 3

Effects of FMNL2 silencing on cell cycle and proliferation markers in MDA-MB-231 and BT549 cells. After transfection for 48 h, cultured cells were processed for indicated assays. a Representative EdU staining images of cells after transfection were shown. b Representative percentage of cells in the G0/G1, G2/M and S phases was detected by flow cytometric analysis. c The levels of FMNL2, p27, p21, CyclinD1, and β-actin proteins were determined by western blotting and representative blots were shown. d The levels of CDK4 and CDK6 mRNA were determined by qRT-PCR. e The CDK4/CyclinD1 kinase activity was determined. Scale bar, 50 μm. *: P < 0.05 vs NC siRNA group

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BMC Cancer

ISSN: 1471-2407

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