Fig. 6

Restore MAP3K13 overexpression or miR-4500 knockdown partially reverses the effects caused by LINC01287 depletion in colon cancer cells. A, SW480 and HCT116 cells were infected with MAP3K13 or empty vector ctrl (Ctrl) lentiviral particles, then protein level of MAP3K13 was tested by western blot. Uncropped gels were in Supplementary Fig. 2. B, SW480 and HCT116 cells were infected with anti-miR-4500 or anti-miR-ctrl lentviral particles, then miR-4500 level was measured by qRT-PCR. C, SW480 and HCT116 cells were infected with sh-ctrl, sh-LINC01287–1, MAPK3K13 or anti-miR-4500 lentiviral particles as indicated, then seeded into 96-well plates (2500/well). Cell viability was tested at indicated time points. D-E, SW480 and HCT116 cells were infected with sh-ctrl, sh-LINC01287–1, MAPK3K13 or anti-miR-4500 lentiviral particles as indicated, then seeded into 6-well plates (1500/well) and grew for 2 weeks for colony formation assay. Representative images for colonies (D) and relative cell confluence of all colonies (E) were shown. F-I, SW480 and HCT116 cells were infected with sh-ctrl, sh-LINC01287–1, MAPK3K13 or anti-miR-4500 lentiviral particles as indicated, then used for transwell migration (F-G) or invasion assay (H-I). Representative images for migration cells (F) or invasion cells (H), and relative migration (G) and invasion (I) cells were shown. Scale bar = 50 μm. J-K. SW480 and HCT116 cells were infected with sh-ctrl, sh-LINC01287–1, MAPK3K13 or anti-miR-4500 lentiviral particles as indicated, then protein expression of indicated genes was tested by western blot (J). Uncropped gels were in Supplementary Fig. 2. Relative protein expression compared with GAPDH was shown (K). L, the potential mode of action of LINC01287 in colon cancer cells. Compared with anti-miR-ctrl or sh-LINC01287–1 group using two-tailed Student’s t-test, *p ≤ 0.05, **p ≤ 0.001