Fig. 4

LINC01287 serves as a molecular sponge for miR-4500 in colon cancer. A-B, SW480 and HCT116 cells were infected with LINC01287 or EV lentiviral particles, then expression levels of indicated microRNAs were tested by qRT-PCR. C, SW480 and HCT116 cells were infected with sh-LINC01287–1, sh-LINC01287–2 or sh-ctrl lentiviral particles, then miR-4500 level was measured by qRT-PCR. D, the predicted binding sites of LINC01287 and miR-4500. E, The pMIRREPORT plasmid containing the wt or mt 3′ UTR of LINC01287, the miR-4500 expression plasmid or miR-ctrl, and a renilla luciferase plasmid (2: 2: 1) were transient transfected into HEK293T cells for luciferase reporter assay. F, the levels of LINC01287 pulled down by biotinylated wt or mt miR-4500, or non-targeting control (NC) were tested by qRT-PCR. G, spearman analysis of correlation between LINC01287 and miR-4500 expression in 60 colon cancer patients. Compared with EV or Bio-NC group using two-tailed Student’s t-test, *p ≤ 0.05, **p ≤ 0.001. n.s. = not significant