Fig. 2

Silencing LINC01287 suppressed proliferation, migration, invasion and EMT of colon cancer cells. A, LINC01287 expression in SW480 and HCT116 cells infected with sh-LINC01287–1, sh-LINC01287–2 or sh-ctrl lentivirus was evaluated by qRT-PCR. B, SW480 and HCT116 cells infected with sh-LINC01287–1, sh-LINC01287–2 or sh-ctrl lentiviral particles were seeded in 96-well plates (2500/well), then cell viability was evaluated at day 0, 2, 4 and 6 post-infection. C-D, SW480 and HCT116 cells infected with sh-LINC01287–1, sh-LINC01287–2 or sh-ctrl lentiviral particles were seeded in 6-well plates (1500/well) and grew for 2 weeks for colony formation assay. Representative images for colonies (C) and relative cell confluence of all colonies were shown (D). E-H, SW480 and HCT116 cells infected with sh-LINC01287–1, sh-LINC01287–2 or sh-ctrl lentiviral particles were used for transwell cell migration (E-F) or invasion (G-H) assay. Representative images for migration cells (E) or invasion cells (G), and relative migration (F) and invasion (H) cells were shown. Scale bar = 50 μm. I-J, SW480 and HCT116 cells infected with sh-LINC01287–1, sh-LINC01287–2 or sh-ctrl lentiviral particles were used for soft agar assay. Representative images (I) and colony numbers per well (J) were shown. Scale bar = 500 μm. K, SW480 and HCT116 cells were infected with sh-LINC01287–1, sh-LINC01287–2 or sh-ctrl lentiviral particles, then expression levels of indicated genes were measured by qRT-PCR. L-M. SW480 and HCT116 cells were infected with sh-LINC01287–1, sh-LINC01287–2 or sh-ctrl lentiviral particles, then protein expression levels of indicated genes were tested by western blot (L). Uncropped gels were in Supplementary Fig. 1. Relative protein expression compared with GAPDH was shown (M). Compared with sh-ctrl group using two-tailed Student’s t-test, *p ≤ 0.05, **p ≤ 0.001