Table 2 Primers designed for qRT-PCR validation of mRNA
From: TBX5-AS1, an enhancer RNA, is a potential novel prognostic biomarker for lung adenocarcinoma
Name | Bidirectional primer sequence | Tm (°C) | Product length (bp) |
|---|---|---|---|
β-actin(H) | F:5′ GTGGCCGAGGACTTTGATTG3’ | 60 | 73 |
R:5′ CCTGTAACAACGCATCTCATATT3’ | |||
TBX5 | F:5′ GGTCTCTTTTGGTGGTCCTTTT 3’ | 60 | 195 |
R:5′ TCAGGCTCCAGAGGCGTGT 3’ |