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Table 1 Primers designed for qRT-PCR validation of eRNA

From: TBX5-AS1, an enhancer RNA, is a potential novel prognostic biomarker for lung adenocarcinoma

Name Bidirectional primer sequence Tm (°C) Product length (bp)
β-actin(H) F:5′ GTGGCCGAGGACTTTGATTG3’ 60 73
R:5′ CCTGTAACAACGCATCTCATATT3’
TBX5-AS1 F:5′ CGCAGTGGTGGATGCTCG 3’ 60 189
R:5′ CTCGGCTCAGAGGTCAAGTAGG 3’