Fig. 8

Profile of cell signalling and cell cycle proteins in the DCIS cell lines compared to breast cancer cell lines. A and B IGF-1R, EGFR and p-EGFR protein level. C ERK, p-ERK, AKT and p-AKT protein levels under normal conditions. D, E and F Comparison of ERK, p-ERK, AKT and p-AKT protein levels under normal conditions and serum-starvation conditions. H and I Cyclin B1 and D1 protein levels. Protein level was quantified and normalized against β actin or GAPDH using ImageJ. Experiments were repeated in triplicate, except the serum-starvation experiments were repeated with four, independent biological replicates. Horizontal bars in panels B, D, G and I indicate the mean protein expression relative to the loading control +/− SEM (GraphPad Prism v.8.3.0). Panels A, C, E, F and H are representative immunoblots. Full-length blots are presented in Supplementary Materials