Fig. 7

ETCC-006 and ETCC-010 display markers of epithelial to mesenchymal (EMT) transition. A Schematic illustrating the markers associated with EMT B Western blot analysis of EMT markers in the DCIS cell lines compared to breast cancer cell lines, MCF7 and MDA-MB-231, and normal-like breast epithelial cells, MCF10A. 40 μg of total protein was loaded per lane onto the gel. C Protein level was quantified and normalized against β actin or GAPDH using ImageJ. This experiment was repeated with four, independent biological replicates (N = 4), panel B is a representative immunoblot. Panel A was created with BioRender.com. Full-length blots are presented in Supplementary Materials