Fig. 2
From: Pyruvate carboxylase promotes thyroid cancer aggressiveness through fatty acid synthesis
Effect of PC expression on lipid accumulation in TC cells. A The intracellular free acid content was quantified by LC/MS. B Western blotting was used to evaluate PC expression in TC cell lines as indicated. C Fatty acid oxidation analysis was detected in 8505C cells by a Seahorse XF24 Extracellular Flux Analyzer. D The cellular content of TAGs was measured in different TC cells. E Nile red dye and DAPI staining were used to detect the neutral lipid content in TC cells by microscopy with a 40× objective lens. F, G 8505C and TPC1 cells were stained with Nile red and subjected to flow cytometry analysis. The results are expressed as means ± standard deviation (SD) of three independent experiments and shown in histograms on the right. * p < 0.05; *** p < 0.01 versus PC WT. Note: LC/MS (liquid chromatography/mass spectrometry), PC (pyruvate carboxylase), TC (thyroid cancer), TAG (tricarboxylic acid cycle)